Key Characteristics:
Chemical Name: 2',4',5',7'-tetrabromofluorescein, also known as Acid Red 87.
Appearance: Typically a brownish-red powder.
Solubility: Water-soluble, meaning it dissolves readily in water.
Use: Primarily used in histology for staining cellular components (cytoplasm, connective tissue).
Staining Properties: Produces a pink to red color in stained tissues.
Other Uses: Can be used in the Papanicolaou stain (Pap stain) and Romanowsky type cytologic stains.
In the Context of Staining:
H&E Stain:
Eosin Y is a crucial component of the H&E stain, a widely used technique in histology for visualizing tissue structures.
Differential Staining:
It's used to differentiate between various cellular and tissue components based on their staining properties.
Staining Mechanism:
Eosin Y is an acidic dye and binds to basic (positively charged) components of cells and tissues, such as proteins in the cytoplasm.