Name: Chocolate Agar Base Dehydrated Culture Media- 500 gm
Brand: TM Media
Price: 100.0 INR
Availability: OutOfStock

Diagnostics Microbiology Reagents Chocolate Agar Base Dehydrated Culture Media

Chocolate agar base is a dehydrated culture medium formulated to support the growth of fastidious bacteria, particularly Haemophilus and Neisseria species. It is a nutrient-rich medium, essentially a variant of blood agar where the red blood cells are lysed (broken down) by heat, releasing essential growth factors like NAD (V factor) and hemin (X factor). This lysis is what gives the medium its characteristic chocolate-brown color

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Neisseria gonorrhoeae is a gram-negative bacteria and the causative agent of gonorrhea, however it is also occasionally found in the throat. The cultivation medium for gonococci should ideally be a rich nutrients base with blood, either partially lysed or completely lysed. The diagnosis and control of gonorrhea have been greatly facilitated by improved laboratory methods for detecting, isolating and studying N. gonorrhoeae.
Chocolate Agar Base, with the addition of supplements, gives excellent growth of the gonococcus without overgrowth by contaminating organisms. G.C. Agar can also be used in place of Chocolate Agar Base, which gives slightly better results than Chocolate Agar. The diagnosis and control of gonorrhea have been greatly facilitated by improved laboratory methods for detecting, isolating and studying N. gonorrhoea.
Interest in the cultural procedure for the diagnosis of gonococcal infection was stimulated by Ruys and Jens, Mcleod and co-workers, Thompson, Leahy and Carpenter, Carpenter, Leahy and Wilson and Carpenter, who clearly demonstrated the superiority of this method over the microscopic technique. Chocolate Agar Base with addition of supplement not only supports the growth of the gonococcus in pure culture but also permits its development from the mixed flora encountered in chronic gonococcal infections. Carpenter reported that this medium and Haemoglobin is useful for cultural detection of the gonococcus.

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